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These conditions can make it difficult for the body to process fluids and nutrients, leading to complications

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For passaging of organoids, the collagen domes were incubated with 1 mg ml 1 collagenase from Clostridium histolyticum (Sigma-Aldrich) for 30 min at 37 C, and digestion monitored using bright-field microscopy (small clumps of cells)

As a result, research and commercial development on the plant was prohibited for most of the 20th century ( Cannabis sativa became the most commonly cultivated black market crop in the Western world after World War II, leading to the allocation of significant law enforcement resources to remove the plants (Chouvy, 2019)

Optogenetic approaches in biotechnology and biomaterials

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