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As expected, the GSH levels were reduced by 100 M of hydrogen peroxide but enhancing network activity with blockers of GABA A receptors (bicuculine) and KCNA channels (4-AP) attenuated this effect

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Further experiments revealed that hydrogen-induced Nrf2 can directly bind to NF-B p65, interfering with its DNA-binding ability to pro-inflammatory gene promoters while also suppressing the release of inflammatory mediators such as HMGB1 and TNF-, thereby truncating the positive feedback loop of IKK