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Understanding the cellular and molecular mechanisms that maintain the structure, function and physiology of the different tissues of the eye is important for identifying key processes that contribute to age related eye diseases such as lens cataract and retinal degeneration

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Pelleted cells were dissolved in 50 mM NaPO 4 , pH 8, 300 mM NaCl, 10 mM imidazole, 0.1% Tween-20, and lysed by sonication

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