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Campolo et al conducted a randomized placebo-controlled trial on 16 male subjects with cardiovascular risk factors

Within the last years, based on the reversible nature of epigenetic marks, small molecules referred to as epidrugs were developed to target epigenetic regulators (Table 1) including 5-azacytidine (5-AZA) and 5-aza-2-deoxycytidine (decitabine), inhibiting DNMTs and inducing a reprogramming of the genome

Genes Dev 21:519524 Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E et al (2004) A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes

Next, 18 l of the soluble polar metabolite supernatant was transferred to sample vials for analysis by LCMS as previously described 4,28

These compounds are also economical, simple to apply, and easily available, as well as show promise for applications in the future

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